Cytometry & Cell sorting

Immune & Tumor cell phenotyping, and sorting of cell subpopulation

Flow cytometry and cell sorting are powerful tools, and provide valuable insights. However, mastering standard and custom panels, dedicated to immune cells, is a massive challenge to secure a proper gating strategy. We are one of the very few experts in EU combining cytometry and cell sorting with NGS libraries preparation and sequencing. This offer a tremendous advantages to researchers to isolate and purified specific cell population, increase sensivity, and integrate various omics in downstream readouts.

Objectives: immune phenotyping, cell cycle or proliferation analysis, purification of specific and are cells, streamlining of NGS sequencing

Starting material: Fresh or frozen samples, from Organ, tumor, Blood and cell suspension.

Flow Cytometry using Standard & Tailored Panels

Standard immunophenotyping: T cell (T cell, Treg, Th); B cell; DCs, monocytes & NK cells; Cell viability & apoptosis assays.

Advanced immunophenotyping: Antigen specific cells; Intracellular staining; Rare cell phenotyping; Cytokine.

Custom immunophenotyping: Tailored panels and Specific staining solution.

Cell Sorting of specific or rare subpopulation, directly used for single cell sequencing

Examples of standard panels:

Examples of standard panels

Live/Dead and CD3

T cells: CD4, CD8, CCR7, CD45RA, CD38, HLA-DR.
T reg cells: CD4, CD25, CD127, CCR4, HLA-DR, CD45RO, Foxp3.
T helper: CD4, CD8, CXCR3, CCR6, CD38, HLA-DR.
B cells: CD24, CD19, CD27, CD38, CD20, IgD.
Myeloid cells: CD11b, CD11c, CD14, CD16, CD33, CD123, PD-L1, HLA-DR.